HuProt™ Human Proteome Microarray

World's Most Comprehensive Collection of Full-length Proteins

With more than 21,000 GST-purified proteins, there has never been a protein library as comprehensive or thoroughly validated as HuProt™.

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The Broadest Coverage of the Human Proteome

HuProt™ consists of more than 21,000 unique human proteins, isoform variants and protein fragments – covering 16,794 unique genes, it is the world's most comprehensive collection of full-length human proteins.

Our industry-leading HuProt microarray includes 15,889 of the 19,613 canonical human proteins described in Human Protein Atlas, with broad coverage across protein subclasses.

Add your own custom proteins to HuProt - on the rare occasion when HuProt does not contain a protein that is of interest to you, we can add that protein(s) for a small additional fee.

Content includes major functional classes such as intracellular proteins, membrane proteins, enzymes, secreted proteins, transcription factors, transporters, GPCRs, cytokines, immune receptors, immune checkpoints, CD markers, ion channels, cytosolic proteins, nuclear receptors. Additionally there is thorough coverage for proteins enriched in major tissues of interest such as testis, cerebral cortex, thyroid gland, skin, fallopian tube, liver, parathyroid, intestine, kidney, spleen, muscle, epididymis, lymph node, bone marrow, adrenal gland, esophagus, heart, appendix, tonsil, prostate, rectum, adipose tissue, stomach, colon, cervix, uterus, gallbladder, seminal vesicle, breast, ovary, endometrium, smooth muscle, salivary gland, pancreas, and bladder.

Unparalleled Content, Reproducibility and Sensitivity

for Autoantibody Discovery

Protein libraries and analysis services for antibody seromics, biomarker discovery, cross-reactivity screening,and other whole-proteome target identification and interaction assay.

Reproducible Protein Distribution

CDI Labs’ non-contact piezoelectric ‘inkjet’ process uses next-generation Arrayjet printers and allows for rapid production of high quality microarray slides time after time. In comparison to older contact pin printing methods – HuProt™ arrays are made with improved accuracy and reproducibility with excellent spot morphology.

Reproducible Serum and Plasma Profiling

Reproducible Proteome-Wide IgG Autoantibody Immunoprofiling of Healthy Human Pooled Plasma Within and Across HuProt Proteome Microarray Batches

HuProt Scatter Diagram Part 1

Pooled human plasma was incubated on pairs of HuProt proteome microarrays across two print batches and stained with a fluorescent anti-IgG secondary antibody. Raw median fluorescence intensity data were captured, log2 transformed, and plotted. Linear regression analysis demonstrates reproducibility across the array pair from Lot 1 (R2 = 0.96), Lot 2 ( R2 = 0.92), and the averaged data from both array batches (R2 = 0.96). These results demonstrate the robust reproducibility of HuProt autoantibody seromics data both within and across microarray production lots.

Full-length Proteins in Their Native Conformation

Protein purification prior to microarray manufacturing selects for proteins with correctly folded GST-fusion proteins. Testing on the HuProt Microarray demonstrates that the proteins are folded in native conformation and retain function (Hu S. poster; Venkataraman A., et al., 2018). When both native and denatured HuProt microarrays were probed with monoclonal antibodies that selectively recognize either linear or folded epitopes of their cognate antigen, the antibodies were found to recognize the appropriate antigen form (Venkataraman A., et al., 2018). Further tests (RNA binding) demonstrated that proteins on HuProt do retain function (Venkataraman A., et al., 2018).

HuProt Focused Arrays

High-throughput Autoantibody Signature Validation

We also offer high-throughput focused array services to validate autoantibody signatures discovered with the HuProt microarray. CONTACT USfor more details on our focused array services.