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Protein-Nucleic Acid Interactions

HuProt enables that understanding of gene regulation and for developing novel therapeutics for diseases.

HuProt Advances the Understanding of Gene Regulation

Systematically characterize the human protein-DNA interactome

Protein-nucleic acid interactions play a fundamental role in various biological processes, including gene expression, DNA replication, transcription, translation, and RNA processing. These interactions involve proteins binding to nucleic acids, such as DNA or RNA, to regulate their function and activity. Understanding protein-nucleic acid interactions and developing strategies to modulate these interactions hold great promise for advancing our understanding of gene regulation and for developing novel therapeutics for various diseases, including cancer, genetic disorders, and infectious diseases.

In the featured publication below, HuProt™ microarray was used as part of a combined bioinformatics and protein microarray-based strategy to systematically characterize the human protein-DNA interactome. The paper identified 17,718 protein-DNA interactions (PDIs) between 460 DNA motifs predicted to regulate transcription and 4,191 human proteins of various functional classes. Many known PDIs for transcription factors (TFs) were recovered along with a large number of unanticipated PDIs for known TFs, as well as for previously uncharacterized TFs.

Featured Publication

Proﬁling the Human Protein-DNA Interactome Reveals ERK2 as a Transcriptional Repressor of Interferon Signaling

Shaohui Hu1, Zhi Xie, Akishi Onishi, Xueping Yu, Lizhi Jiang, Jimmy Lin, Hee-sool Rho, Crystal Woodard, Hong Wang, Jun-Seop Jeong, Shunyou Long, Xiaofei He, Herschel Wade, Seth Blackshaw, Jiang Qian, Heng Zhu1

Summary

Protein-DNA interactions (PDIs) mediate a broad range of functions essential for cellular differentiation, function, and survival. However, it is still a daunting task to comprehensively identify and profile sequence-specific PDIs in complex genomes. Here, we have used a combined bioinformatics and protein microarray-based strategy to systematically characterize the human protein-DNA interactome. We identified 17,718 PDIs between 460 DNA motifs predicted to regulate transcription and 4,191 human proteins of various functional classes.

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CDI Labs Advantages

The most comprehensive protein collections available allow for rapid proteome wide antibody repertoire analysis and target identification studies.

HuProt™ Proteome Microarrays provide high quality, reproducible results with a protein purification process that only selects properly folded proteins.

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