MouseScan™ Murine PhIP-Seq: Decode the Complete Immune History of Mouse Models | CDI Labs

Complete Murine Proteome Autoantibody Profiling with Epitope-level Precision

Phage ImmunoPrecipitation Sequencing (PhIP-Seq) is a powerful method of multiplexed analysis that combines DNA high-throughput sequencing with next-generation proteomics

Imagine mapping your mouse model\'s complete immune history in a single tube. MouseScan PhIP-Seq revolutionizes autoantibody research by analyzing the entire murine proteome, over 50,000 proteins and isoforms simultaneously. While traditional methods test for a handful of known targets, MouseScan uses cutting-edge Phage ImmunoPrecipitation Sequencing (PhIP-Seq) to detect antibodies against virtually any mouse antigen with epitope-level precision. This breakthrough technology transforms weeks of individual assays into one comprehensive analysis, giving researchers unprecedented insights into immune responses, autoimmune disease mechanisms, and therapeutic targets they never knew existed.

PhIP-Seq allows researchers to determine what antibodies were created by a mouse\'s unique history of immune exposures. PhIP-Seq is the highest-coverage antigen-specific assay yet developed, able to detect antibodies versus virtually any antigen via overlapping long peptides. MouseScan enables simultaneous epitope-level autoantibody profiling versus an entire GRCm38.p5 mouse proteome (50,135 proteins and isoforms) via phage immunoprecipitation sequencing.

MouseScan PhIP-Seq is composed of the entire normal mouse proteome (GRCm38.p5). The library contains all proteins expressed as overlapping 62-mer peptide tiles on the surface of bacteriophages. For an assay, an aliquot from this library is reacted with diluted mouse serum or other antibody-containing fluid. Bound antibodies are immunoprecipitated with protein A/G beads, the precipitate amplified by PCR, and the sequences quantified by a next-generation sequencing and analysis pipeline that compares patient-sample IP read counts to negative controls with no antibody input (mock IPs) in the context of overall clonal frequency of individual peptides in the parent library. Output data are then created at both the peptide level.

MouseScan Technology Overview

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What Our Customers Are Saying

"With CDI Labs, identifying antibodies and their targets has never been so simple. Their passionate team, robust science and high-quality service unlock the discovery of new mechanisms, biomarkers and therapeutic targets."

Matthieu Roulleaux DugageGustave Roussy View poster, "Baseline serum level of IgG targeting annexin A2 is predictive of response to immunotherapy in patients with advanced NSCLC" presented at IMMUNOLOGY 2025

What Our Customers Are Saying

"We enjoyed working with CDI Labs. We used both VirScan and HuScan services from CDI Labs as part of our neurodegenerative disease research. The team at CDI Labs was very responsive and great to work with, and they went above and beyond to get us our results very quickly. They were particularly helpful in making contract arrangements and ensuring that everything was in place for a smooth collaboration. CDI Labs also provided a comprehensive statistical analysis, which will be invaluable as we build on our research. We’re still analyzing the data, but we’re confident that we’ll gain new insights into disease mechanisms that will advance our research."

Wouter Peelaerts, PhDProfessor, KU Leuven

What Our Customers Are Saying

"CDI Labs provide consistent service and consistently high-quality protein array products to our lab. Their sales and scientific team are courteous and timely in their responses to our queries.”

Dr. Andrew McKeonMayo Clinic College of Medicine & Science

Data and Technology Validated by Scientists

"We elected to use the HuProt Microarray because it is an extensive platform that contains over 21,000 unique, individually purified full-length human proteins and protein isoforms in duplicate, covering more than 81% of the proteome."

OncologistSchool of Medicine Research (as mentioned in "Baseline Serum Autoantibody Signatures Predict Recurrence and Toxicity in Melanoma Patients Receiving Adjuvant Immune Checkpoint Blockade" - AACR Clinical Caner Research - September 2022)

Data and Technology Validated by Scientists

"This (VirScan) assay, which uses phage display immunoprecipitation and sequencing, is a sensitive and focused high-comprehensive approach that enables thorough serological profiling of antiviral antibodies in humans and, consequently, the identification of viral exposure throughout the human virome."

Faculty of MedicineResearch Institution (as mentioned in "Human virome profiling identified CMV as the major viral driver of a high accumulation of senescent CD8+ T cells in patients with advanced NSCLC" - Science Advances - November 2023)

Data and Technology Validated by Scientists

"By using (HuProt) protein arrays, we were able to evaluate a broader range of antigens compared to previous investigations."

Senior ScientistBiotech Research (poster presentation at the San Antonio Breast Cancer Symposium 2023)

Related Resources

Content

ZIP | 16.98 MB

MouseScan PhIP-Seq Content

An entire mouse proteome (GRCm38.p5) is included in MouseScan autoantibody testing. Zip file containing .csv...

Get Unprecedented Insights Into Disease Progression and Mechanisms via Murine Models

Complete Murine Proteome Autoantibody Profiling with Epitope-level Precision

Phage ImmunoPrecipitation Sequencing (PhIP-Seq) is a powerful method of multiplexed analysis that combines DNA high-throughput sequencing with next-generation proteomics

MouseScan Technology Overview

Ready to Transform Your Research?

What Our Customers Are Saying

"With CDI Labs, identifying antibodies and their targets has never been so simple. Their passionate team, robust science and high-quality service unlock the discovery of new mechanisms, biomarkers and therapeutic targets."

What Our Customers Are Saying

What Our Customers Are Saying

"CDI Labs provide consistent service and consistently high-quality protein array products to our lab. Their sales and scientific team are courteous and timely in their responses to our queries.”

Data and Technology Validated by Scientists

"We elected to use the HuProt Microarray because it is an extensive platform that contains over 21,000 unique, individually purified full-length human proteins and protein isoforms in duplicate, covering more than 81% of the proteome."

Data and Technology Validated by Scientists

Data and Technology Validated by Scientists

"By using (HuProt) protein arrays, we were able to evaluate a broader range of antigens compared to previous investigations."

Related Resources

MouseScan PhIP-Seq Content

Overview of Autoantibody Seromics